Synthetic Biology for Actinomycetes
In Actinomycetes, the tools of Synthetic Biology comprise several strategies to build a chassis for high-yield producer strains of heterologously expressed biomolecules.
Reduced genomes by surgical deletion of several biosynthetic gene clusters and protein degrading machinery provides strains with lightened burden, without unnecessary metabolic routes for the bacterium function. Engineering of synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements and/or genes allow better control of expression. Improvement of transcription control by removing sigma factors helps to minimize maintenance efforts and increase product yields. Temporal and product-saving control of gene expression is reached through synthetic promoters and regulatory networks.